{"id":4904,"date":"2021-08-17T11:21:41","date_gmt":"2021-08-17T03:21:41","guid":{"rendered":"https:\/\/www.cdjrs.com\/?p=4904"},"modified":"2021-08-17T11:21:41","modified_gmt":"2021-08-17T03:21:41","slug":"pcr%e5%ae%9e%e9%aa%8c%e5%ae%a4%e5%bb%ba%e8%ae%be%e6%89%80%e4%ba%a7%e7%94%9f%e7%9a%84%e6%b1%a1%e6%9f%93%e6%ba%90%e4%b8%bb%e8%a6%81%e6%9c%89%e5%93%aa%e4%ba%9b%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.cdjrs.com\/4904.html","title":{"rendered":"PCR\u5b9e\u9a8c\u5ba4\u5efa\u8bbe\u6240\u4ea7\u751f\u7684\u6c61\u67d3\u6e90\u4e3b\u8981\u6709\u54ea\u4e9b\uff1f"},"content":{"rendered":"

PCR\u5b9e\u9a8c\u5ba4\u5c5e\u4e8e\u4e09\u7ea7\u751f\u7269\u5b89\u5168\u5b9e\u9a8c\u5ba4\uff0c\u5176\u4ea7\u751f\u7684\u6c61\u67d3\u6e90\u4e3b\u8981\u6709\u54ea\u4e9b?\u4f60\u4e86\u89e3\u591a\u5c11\uff0c\u4e00\u8d77\u6765\u770b\u770b\u5427\u3002<\/p>\n

PCR\u5b9e\u9a8c\u5ba4\u5e38\u89c1\u6c61\u67d3\u7269\u6709\u4ee5\u4e0b\u4e94\u79cd:(1)\u4e34\u5e8a\u6837\u672c\u4e2d\u542b\u6709\u5927\u91cf\u5f85\u6d4b\u5fae\u751f\u7269\uff1b(2)\u901a\u8fc7\u79d1\u5b66\u7814\u7a76\u83b7\u5f97\u8d28\u7c92\u514b\u9686\uff1b(3)\u4ee5\u524d\u5206\u6790\u7814\u7a76\u7684\u7279\u5b9a\u5fae\u751f\u7269\uff1b(4)\u5b9e\u9a8c\u73af\u5883\u4e2d\u542b\u6709\u5927\u91cf\u7279\u6b8a\u5fae\u751f\u7269\uff1b(5)\u524d\u671f\u6269\u589e\u4ea7\u54c1\u7684\u6b8b\u7559\u6c61\u67d3\u3002<\/p>\n

\u5728 PCR\u5b9e\u9a8c\u5ba4\uff0c\u8fd9\u4e5f\u662f\u6700\u5bb9\u6613\u4ea7\u751f\u5047\u9633\u6027\u7684\u6c61\u67d3\u7269\u3002\u901a\u5e38\uff0c\u4e00\u4e2a\u5178\u578b\u7684 PCR\u53cd\u5e94\u53ef\u4ee5\u751f\u621010^9\u4e2a\u91cd\u590d\u7684\u5e8f\u5217\uff0c\u8fd9\u6837\u4e00\u4e2a PCR\u53cd\u5e94\u5c31\u53ef\u4ee5\u4ea7\u751f10^9\u4e2a\u91cd\u590d\u7684\u5e8f\u5217\uff0c\u5728\u6c14\u6eb6\u80f6\u4e2d\uff0c\u5373\u4f7f\u662f\u6700\u5c0f\u7684\u6c14\u6eb6\u80f6\uff0c\u4e5f\u53ef\u4ee5\u5f97\u523010^6\u7684\u6269\u589e\u4ea7\u7269\u3002\u5982\u679c\u4e0d\u52a0\u4ee5\u63a7\u5236\uff0c\u6c14\u6eb6\u80f6\u5316\u53cd\u5e94\u7269\u5728\u77ed\u671f\u5185\u4f1a\u6c61\u67d3\u5b9e\u9a8c\u5ba4\u8bd5\u5242\uff0c
\n\u4eea\u8868\u4e0e\u901a\u98ce\u7cfb\u7edf\u4f1a\u9020\u6210\u4e25\u91cd\u7684\u5b9e\u9a8c\u5ba4\u6c61\u67d3\uff0c\u5e76\u4ea7\u751f\u5927\u91cf\u5047\u9633\u6027\u7ed3\u679c\u3002RNA\u6269\u589e\u6280\u672f\uff0c\u5982 NASBA (NASBA)\uff0c\u8f6c\u5f55\u4ecb\u5bfc\u6269\u589e\u3002
\n\u4e0d\u5bb9\u6613\u4ea7\u751f\u6269\u589e\u4ea7\u7269(TMA)\u3001 Qbeta\u590d\u5236\u9176\u7b49\uff0c\u5176\u6269\u589e\u4ea7\u7269\u4e3a RNA\uff0c\u5728\u73af\u5883\u4e2d\u5feb\u901f\u964d\u89e3\u3002\"PCR\u5b9e\u9a8c\u5ba4\u5efa\u8bbe\u6240\u4ea7\u751f\u7684\u6c61\u67d3\u6e90\u4e3b\u8981\u6709\u54ea\u4e9b\uff1f\u63d2\u56fe\"<\/p>\n

\n2.\u6c61\u67d3\u9884\u9632\u63aa\u65bd
\n(1)\u4e25\u683c\u5212\u5206\u5b9e\u9a8c\u5ba4\uff0c\u4e25\u683c\u9075\u5b88
\n\u8bd5\u9a8c\u5ba4\u5206\u4e3a\u8bd5\u5242\u51c6\u5907\u533a\u3001\u6837\u54c1\u9884\u5236\u533a\u3001 PCR\u6269\u589e\u533a\u3001\u4ea7\u7269\u5206\u6790\u533a\u7b49\uff0c\u5e76\u914d\u5907\u76f8\u5e94\u7684\u4eea\u5668\u8bbe\u5907\u3001\u5de5\u4f5c\u670d\u3001\u624b\u5957\u3001\u53d6\u6837\u88c5\u7f6e\u548c\u901a\u98ce\u7cfb\u7edf\u3002\u5185\u90e8\u533a\u57df
\n\u5fc5\u987b\u76f4\u63a5\u5728\u5404\u81ea\u5730\u533a\u7ec4\u88c5\u6216\u5305\u88c5\u4f7f\u7528\u8bd5\u5242\u548c\u5e9f\u7269\u3002\u64cd\u4f5c\u8005\u5fc5\u987b\u5c0f\u5fc3\u9632\u6b62\u4ece\u6c61\u67d3\u533a\uff0c\u5305\u62ec\u5934\u53d1\u3001\u773c\u775b\u548c\u8863\u670d\u7b49\u88ab\u5e26\u5165\u6e05\u6d01\u533a\u57df\u3002<\/p>\n

(2\uff09\u5316\u5b66\u6e05\u7406
\n\u8bd5\u9a8c\u53f0\u9762\u4e0a\u53ef\u752810%\u6b21\u6c2f\u9178\u94a0(\u6f02\u767d\u5242)\u6e05\u6d17\uff0c\u518d\u752870%\u4e59\u9187\u6216\u6e05\u6c34\u6d17\u53bb\u6b21\u6c2f\u9178\u94a0\u3002\u6b21\u6c2f\u9178\u94a0\u53ef\u4ee5\u6c27\u5316\u635f\u4f24\u6838\u9178\uff0c\u56e0\u6b64\u53ef\u4ee5\u505c\u7559\u5728\u8bd5\u9a8c\u53f0\u8868\u9762
\n\u5206\u6563\u7684\u4ea7\u7269\u88ab\u6467\u6bc1\u3002\u7ed3\u679c\u8868\u660e\uff0c\u6b21\u6c2f\u9178\u94a0\u5bf9\u63d0\u53d6\u7684 DNA\u548c PCR\u6269\u589e\u4ea7\u7269\u5747\u65e0\u6cd5\u533a\u5206\uff0c\u800c\u7528\u6b21\u6c2f\u9178\u94a0\u5904\u7406\u7684\u6837\u54c1\u65e0\u6cd5\u8fdb\u884c\u6838\u9178\u6269\u589e\u68c0\u6d4b\u3002\u5b9e\u9645\u4e0a\uff0c\u67d0\u4e9b\u9879\u76ee\u7684\u5b9e\u9645\u6bd4\u7387
\n\u5982\u679c\u9700\u8981\u8fdb\u5165\u53cd\u5e94\u7ba1\u7684\u5706\u76d8\u9700\u8981\u4ece\u6c61\u67d3\u533a\u8f6c\u5230\u6e05\u6d01\u533a\uff0c\u9700\u8981\u75282-10%\u6b21\u6c2f\u9178\u94a0\u6eb6\u6db2\u6d78\u6ce1\u4e00\u591c\uff0c\u7136\u540e\u5145\u5206\u51b2\u6d17\u3002<\/p>\n

\n(3)\u6269\u5c55\u4ea7\u54c1\u6539\u6027
\n\u4e34\u5e8a PCR\u68c0\u6d4b\u901a\u5e38\u5305\u62ec3~4\u4e2a\u6b65\u9aa4\uff1a\u5236\u5907\u6838\u9178\u63d0\u53d6\u548c\u6269\u589e\u68c0\u6d4b\u8bd5\u5242\u3002\u6837\u54c1\u5904\u7406\u662f\u4e00\u79cd\u63d0\u53d6\u76ee\u6807\u6838\u9178\u3001 PCR\u6269\u589e\u3001\u4ea7\u7269\u6269\u589e\u7b49\u6280\u672f\uff0c\u662f\u4e00\u79cd\u65b0\u7684\u68c0\u6d4b\u65b9\u6cd5\u3002PCR\u65b9\u6cd5\u540c\u65f6\u8fdb\u884c PCR\u6269\u589e\u548c\u6269\u589e\u4ea7\u7269\u5206\u6790\u3002
\n\u5bf9\u4ee5\u524d\u7684\u6269\u589e\u4ea7\u7269\u8fdb\u884c\u9002\u5f53\u7684\u4fee\u9970\uff0c\u65b0\u7684\u6269\u589e\u68c0\u6d4b\u5e94\u8be5\u80fd\u88ab\u6392\u9664\uff0c\u4f46\u662f\u4e3a\u4e86\u4fdd\u8bc1\u6269\u589e\u68c0\u6d4b\u4e0d\u4f1a\u5f71\u54cd\u9776\u6838\u9178\uff0c\u6211\u4eec\u5fc5\u987b\u9075\u5faa\u4e24\u4e2a\u57fa\u672c\u539f\u5219\uff1a
\n\u4ea7\u7269\u7ecf\u4fee\u9970\u540e\uff0c\u53ef\u4e0e\u6269\u589e\u53cd\u5e94\u7ba1\u8fdb\u884c\u4fee\u9970\uff0c\u5e76\u53ef\u4e0e\u6269\u589e\u53cd\u5e94\u7ba1\u76f8\u533a\u522b\uff0c\u4f46\u4e0d\u5f71\u54cd\u6269\u589e\u4ea7\u7269\u5e8f\u5217\u3002
\n\u524d\u5904\u7406\uff1a\u7d2b\u5916\u5149\u7167\u5c04\uff0c\u9176\u5904\u7406\u3002
\n\u4fee\u590d\uff1a\u8865\u9aa8\u8102\u7d20\u3001\u5f02\u8865\u9aa8\u8102\u7d20\u3001\u76d0\u9178\u7f9f\u80fa\u3001\u5f15\u7269\u6c34\u89e3\u7b49\u3002<\/p>\n

\u6210\u90fd\u6770\u745e\u601d\u4e13\u6ce8\u4e8e\u6574\u4f53\u5b9e\u9a8c\u5ba4\u5efa\u8bbe\u4e0e\u8bbe\u8ba1\uff0c\u6b22\u8fce\u9009\u62e9\u6211\u4eec\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

PCR\u5b9e\u9a8c\u5ba4\u5c5e\u4e8e\u4e09\u7ea7\u751f\u7269\u5b89\u5168\u5b9e\u9a8c\u5ba4\uff0c\u5176\u4ea7\u751f\u7684\u6c61\u67d3\u6e90\u4e3b\u8981\u6709\u54ea\u4e9b?\u4f60\u4e86\u89e3\u591a\u5c11\uff0c\u4e00\u8d77\u6765\u770b\u770b\u5427\u3002 PCR\u5b9e\u9a8c\u5ba4\u5e38\u89c1\u6c61\u67d3\u7269\u6709\u4ee5 […]<\/p>\n","protected":false},"author":1,"featured_media":4841,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[116,71,115],"_links":{"self":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts\/4904"}],"collection":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/comments?post=4904"}],"version-history":[{"count":1,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts\/4904\/revisions"}],"predecessor-version":[{"id":4905,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts\/4904\/revisions\/4905"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/media\/4841"}],"wp:attachment":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/media?parent=4904"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/categories?post=4904"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/tags?post=4904"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}