{"id":5009,"date":"2021-09-01T09:01:49","date_gmt":"2021-09-01T01:01:49","guid":{"rendered":"https:\/\/www.cdjrs.com\/?p=5009"},"modified":"2021-09-01T09:01:49","modified_gmt":"2021-09-01T01:01:49","slug":"%e5%ae%9e%e9%aa%8c%e5%ae%a4%e8%a3%85%e4%bf%ae%e8%ae%be%e8%ae%a1%e4%b8%8e%e5%bb%ba%e8%ae%be%e8%a6%81%e6%b1%82%e6%b3%a8%e6%84%8f%e4%ba%8b%e9%a1%b9%e6%9c%89%e5%93%aa%e4%ba%9b%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.cdjrs.com\/5009.html","title":{"rendered":"\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u8bbe\u8ba1\u4e0e\u5efa\u8bbe\u8981\u6c42\u6ce8\u610f\u4e8b\u9879\u6709\u54ea\u4e9b\uff1f"},"content":{"rendered":"

\u6770\u745e\u601dPCR\u5b9e\u9a8c\u5ba4\u662f\u6307\u7684\u662f\u57fa\u56e0\u6269\u589e\u5b9e\u9a8c\uff0c\u5176\u7279\u70b9\u662f\u80fd\u5c06\u5fae\u91cf\u7684DNA\u5927\u5e45\u589e\u52a0\uff0c\u662f\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u548c\u5b9e\u9a8c\u7684\u5e38\u89c4\u65b9\u6cd5\uff0cPCR\u5b9e\u9a8c\u5ba4\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u5b66\u5404\u4e2a\u9886\u57df\uff0c\u4f8b\u5982\uff1a\u827e\u6ecb\u75c5\u68c0\u6d4b\uff0c\u4e59\u578b\u809d\u708e\uff0c\u79bd\u75ab\u75c5\uff0c\u764c\u57fa\u56e0\u7684\u68c0\u6d4b\u548c\u8bca\u65ad\uff0cDNA\u6307\u7eb9\uff0c\u4e2a\u4f53\u8bc6\u522b\uff0c\u4eb2\u5b50\u9274\u5b9a\u53ca\u6cd5\u533b\u7269\u8bc1\u7b49\u7b49\u3002<\/p>\n

\u6770\u745e\u601d\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u4eca\u5929\u5c31\u4e3a\u8fd9\u4e2a\u5927\u5bb6\u641e\u4e0d\u61c2\u7684PCR\u5b9e\u9a8c\u5ba4\u8bbe\u8ba1\u3001\u5efa\u8bbe\u8981\u6c42\u548c\u8981\u70b9\u8fdb\u884c\u8be6\u89e3\u3002<\/p>\n

\"\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u8bbe\u8ba1\u4e0e\u5efa\u8bbe\u8981\u6c42\u6ce8\u610f\u4e8b\u9879\u6709\u54ea\u4e9b\uff1f\u63d2\u56fe\"<\/p>\n

\u6210\u90fd\u5b9e\u9a8c\u5ba4\u88c5\u4fee<\/p>\n

\u4e00\u3001PCR\u5b9e\u9a8c\u5ba4\u5e03\u5c40<\/h4>\n

PCR\u5b9e\u9a8c\u5ba4\u539f\u5219\u4e0a\u6709\u56db\u4e2a\u72ec\u7acb\u7684\u5de5\u4f5c\u533a\u57df\uff0c\u6837\u54c1\u5236\u5907\u533a\u3001\u6269\u589e\u4ea7\u7269\u5206\u6790\u533a\uff0c\u5e76\u6709\u4e13\u7528\u8d70\u5eca\uff0c\u6bcf\u4e00\u4e2a\u5de5\u4f5c\u533a\u57df\u5e94\u6709\u7f13\u51b2\u95f4\uff0c\u5de5\u4f5c\u533a\u4e0e\u7f13\u51b2\u95f4\u5b9c\u91c7\u7528\u8fde\u9501\u88c5\u7f6e\u3002\u6bcf\u4e00\u529f\u80fd\u5de5\u4f5c\u533a\u5e94\u5206\u5f00\uff0c\u6bcf\u4e00\u533a\u57df\u6709\u660e\u663e\u7684\u6807\u5fd7\uff0c\u4e0d\u53ef\u76f4\u901a\uff0c\u5982\u8fde\u63a5\u7d27\u5bc6\uff0c\u9700\u5b89\u88c5\u7269\u54c1\u4f20\u9001\u7a97\u3002\u524d\u4e24\u4e2a\u533a\u662f\u6269\u589e\u7684\u524d\u533a\uff0c\u540e\u9762\u4e24\u4e2a\u662f\u6269\u589e\u540e\u7684\u533a\u57df\uff0c\u5373\u4ece\uff1a\u8bd5\u5242\u50a8\u5907\u533a- PCR\u6269\u589e\u5ba4-\u4ea7\u7269\u5ba4\uff0c\u4e0d\u80fd\u53cd\u5411\u6d41\u52a8\u3002\u623f\u95f4\u5185\u7684\u6c14\u6d41\u4e5f\u5e94\u7531\u6269\u5f20\u524d\u533a\u5411\u540e\u6269\u5c55\uff0c\u4e0d\u53ef\u9006\u5411\u6d41\u52a8\u3002\u6bcf\u4e00\u5de5\u4f5c\u533a\u7684\u9876\u90e8\u5e94\u5b89\u88c5\u7d2b\u5916\u706f\uff0c\u6ce2\u957f\u4e3a254\u6beb\u7c73\uff0c\u6bcf20\u33a1\u4e00\u76cf40 W\u7684\u7d2b\u5916\u7ebf\u706f\u3002<\/p>\n

\"\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u8bbe\u8ba1\u4e0e\u5efa\u8bbe\u8981\u6c42\u6ce8\u610f\u4e8b\u9879\u6709\u54ea\u4e9b\uff1f\u63d2\u56fe1\"<\/p>\n

\u5b9e\u9a8c\u5ba4\u5efa\u8bbe<\/p>\n

\u4e8c\u3001 PCR\u5b9e\u9a8c\u5ba4\u5e03\u5c40<\/h4>\n

\u5b9e\u9a8c\u5ba4\u533a\u57df\u529f\u80fd\u548c\u4e3b\u8981\u8bbe\u5907<\/p>\n

1.\u8bd5\u5242\u51c6\u5907\u533a\uff1a\u4e3b\u8981\u8fdb\u884c\u8bd5\u5242\u7684\u914d\u5236\u3001\u5206\u88c5\u53ca\u4e3b\u53cd\u5e94\u6df7\u5408\u7269\u7684\u914d\u5236\u3002\u7528\u4e8e\u5236\u9020\u6837\u54c1\u7684\u8bd5\u5242\u76d2\u6750\u6599\u5e94\u76f4\u63a5\u8fd0\u81f3\u73b0\u573a\u3002\u8bd5\u5242\u7684\u539f\u6599\u5fc5\u987b\u50a8\u5b58\u5728\u672c\u533a\u5185\uff0c\u5e76\u5728\u672c\u533a\u5185\u914d\u5236\u6240\u9700\u7684\u8bd5\u5242\u3002\u672c\u533a\u4e3b\u8981\u8bbe\u5907\u6709\u5929\u5e73\u3001\u51b0\u7bb1\u3001\u79bb\u5fc3\u673a\u3001\u52a0\u6837\u5668\u3001\u632f\u8361\u5668\u7b49\u3002\u5bf9\u4e8e\u98ce\u538b\u7684\u63a7\u5236\uff0c\u672c\u533a\u65e0\u4e25\u683c\u8981\u6c42\uff0c\u4e00\u822c\u662f+10 Pa\u3002<\/p>\n

2.\u6837\u54c1\u51c6\u5907\u533a\u57df\uff1a\u4e3b\u8981\u6837\u54c1\u7684\u4fdd\u5b58\u3001\u6838\u9178\u63d0\u53d6\u3001\u8d2e\u5b58\u3001\u52a0\u5165\u53cd\u5e94\u7ba1\u3001 DNA\u5408\u6210\u6d4b\u5b9a\u3002\u516c\u53f8\u4e3b\u8981\u751f\u4ea7\u7535\u51b0\u7bb1\u3001\u751f\u7269\u5b89\u5168\u67dc\u3001\u79bb\u5fc3\u52a0\u6837\u5668\u3001\u632f\u8361\u5668\u7b49\u3002\u8fd9\u4e00\u533a\u57df\u7684\u538b\u529b\u68af\u5ea6\u8981\u6c42\u662f\uff1a\u901a\u5e38\uff0c\u76f8\u90bb\u533a\u57df\u5e94\u6b63\u538b\uff0c\u907f\u514d\u90bb\u8fd1\u5730\u533a\u8fdb\u5165\u6c61\u67d3\u533a\uff0c\u4e00\u822c\u4e3a+10 Pa\u3002<\/p>\n

3.\u6269\u5c55\u533a\uff1a\u4e3b\u8981\u4e3a DNA\u3002\u6b64\u5916\uff0c\u4e5f\u53ef\u5728\u672c\u533a\u8fdb\u884c\u5236\u5907\u7684 DNA\u6a21\u677f(\u4ece\u6837\u54c1\u5236\u5907\u533a\u57df\u6dfb\u52a0)\u548c\u4e3b\u53cd\u5e94\u6df7\u5408\u6db2(\u4ece\u8bd5\u5242\u9884\u5907\u533a)\u7684\u5236\u5907\u53d8\u6210\u53cd\u5e94\u6df7\u5408\u7269\u3002\u672c\u4ea7\u54c1\u4e3b\u8981\u5305\u62ec\uff1a\u6269\u589e\u4eea\u3001\u51b0\u7bb1\u3001\u79bb\u5fc3\u673a\u3001\u8fdb\u6837\u5668\u3002\u672c\u533a\u57df\u7684\u538b\u529b\u68af\u5ea6\u8981\u6c42\u4e3a\uff1a\u5728\u8be5\u533a\u57df\u5185\uff0c\u538b\u529b\u68af\u5ea6\u4e0e\u90bb\u8fd1\u533a\u57df\u5448\u8d1f\u538b\u5173\u7cfb\uff0c\u907f\u514d\u4e86\u6c14\u6eb6\u73b0\u8c61\u3002<\/p>\n

4.\u4ea7\u7269\u5206\u6790\u9886\u57df\uff1a\u4ea7\u7269\u7684\u6d4b\u5b9a\u3002\u672c\u533a\u4e3b\u8981\u8bbe\u5907\u6709\u9176\u6807\u4eea\u3001\u6d17\u677f\u673a\u3001\u6c34\u69fd\u7b49\u3002\u672c\u533a\u57df\u8981\u6c42\u538b\u529b\u68af\u5ea6\u4e3a-25 Pa\uff0c\u4e00\u822c\u4e3a-25 Pa\uff0c\u8d1f\u538b\u76f8\u5bf9\u4e8e\u90bb\u8fd1\u533a\u57df\uff0c\u4ee5\u907f\u514d\u4ea7\u54c1\u4ece\u8be5\u5730\u533a\u6269\u6563\u5230\u5176\u4ed6\u5730\u533a<\/p>\n

\"\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u8bbe\u8ba1\u4e0e\u5efa\u8bbe\u8981\u6c42\u6ce8\u610f\u4e8b\u9879\u6709\u54ea\u4e9b\uff1f\u63d2\u56fe2\"<\/p>\n

\u6210\u90fd\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u6848\u4f8b<\/p>\n

\u4e09. PCR\u5b9e\u9a8c\u5ba4\u901a\u98ce\u7cfb\u7edf\u53ca\u538b\u529b\u63a7\u5236<\/h4>\n

\u5404\u533a\u57df\u4e4b\u95f4\u5e94\u5177\u5907\u5355\u95f4\u7684\u5b9e\u9a8c\u5de5\u827a\u6d41\uff0c\u7269\u6d41\uff0c\u4eba\u6d41\u4e0e\u6c14\u6d41\uff0c\u5f62\u6210\u5355\u5411\u6d41\u7a0b\u7684\u4fdd\u62a4\u5c4f\u969c\uff0c\u907f\u514d\u5b9e\u9a8c\u5ba4\u4e4b\u95f4\u7684\u76f8\u4e92\u5e72\u6270\uff0c\u9632\u6b62\u6c14\u6eb6\u80f6\u6269\u6563\u5bf9\u5b9e\u9a8c\u8fc7\u7a0b\u9020\u6210\u6c61\u67d3\u3002<\/p>\n

PCR\u5b9e\u9a8c\u5ba4\u5e76\u6ca1\u6709\u4e25\u683c\u7684\u51c0\u5316\u8981\u6c42\uff0c\u4f46\u662f\u4e3a\u907f\u514d\u5404\u4e2a\u5b9e\u9a8c\u533a\u57df\u4ea4\u53c9\u6c61\u67d3\u7684\u53ef\u80fd\u6027\uff0c\u5b9c\u91c7\u7528\u5168\u9001\u5168\u6392\u7684\u6c14\u6d41\u7ec4\u7ec7\u5f62\u5f0f\uff0c\u4e25\u683c\u63a7\u5236\u9001\uff0c\u6392\u98ce\u7684\u6bd4\u4f8b\u4ee5\u4fdd\u8bc1\u4e2a\u5b9e\u9a8c\u533a\u7684\u538b\u529b\u8981\u6c42\u3002<\/p>\n

\u56db.PCR\u5b9e\u9a8c\u5ba4\u88c5\u9970<\/h4>\n

\u5b9e\u9a8c\u5ba4\u56f4\u62a4\u7ed3\u6784\u5e94\u7262\u56fa\uff0c\u6c14\u5bc6\u6027\u597d\uff1b\u6240\u6709\u9634\u9633\u89d2\u5b9c\u91c7\u7528\u5706\u5f27\u8fc7\u5ea6\uff1b\u5899\u4f53\u5185\u58c1\u5149\u6d01\uff0c\u4e0d\u79ef\u5c18\uff0c\u8010\u8150\u8680\uff0c\u6613\u6e05\u6d17\u6d88\u6bd2\uff1b\u5730\u9762\u4f7f\u7528PVC\u5377\u6750\u6216\u73af\u6c27\u6811\u8102\u81ea\u6d41\u576a\uff0c\u6750\u6599\u5e94\u6ee1\u8db3\u65e0\u7f1d\u9699\uff0c\u65e0\u6e17\u6f0f\uff0c\u5149\u6d01\uff0c\u8010\u8150\u8680\u7684\u8981\u6c42\u3002<\/p>\n

\u4e94.PCR\u5b9e\u9a8c\u5ba4\u6ce8\u610f\u4e8b\u9879<\/h4>\n

1.\u51e0\u4e2a\u533a\u57df\u7684\u5927\u5c0f\u8bbe\u7f6e\u60c5\u51b5\uff0c\u8bd5\u5242\u51c6\u5907\u533a\uff0c\u6269\u589e\u533a\uff0c\u6269\u589e\u4ea7\u7269\u5206\u6790\u533a\uff0c\u7a7a\u95f4\u53ef\u4ee5\u76f8\u5bf9\u5c0f\u4e00\u4e9b\uff0c\u6837\u54c1\u5236\u5907\u533a\u8981\u653e\u7f6e\u751f\u7269\u5b89\u5168\u67dc\u548c\u4f4e\u6e29\u51b0\u7bb1\uff0c\u7a7a\u95f4\u5e94\u5927\u4e00\u4e9b\u3002<\/p>\n

2.\u8bd5\u5242\u51c6\u5907\u533a\uff0c\u6837\u54c1\u5236\u5907\u533a\u8bbe\u7d27\u6025\u6d17\u773c\u5668\uff0c\u4ee5\u4fdd\u8bc1\u64cd\u4f5c\u4eba\u5458\u7684\u5b89\u5168\u3002<\/p>\n

\u6210\u90fd\u6770\u745e\u601d\uff1a<\/strong>\u5b9e\u9a8c\u5ba4\u88c5\u4fee\u8bbe\u8ba1\u670d\u52a1\u4e3a\u60a8\u63d0\u4f9b\u4ece\u5b9e\u9a8c\u5ba4\u89c4\u5212\u5e03\u5c40\u5230\u65bd\u5de5\u5efa\u8bbe\u5230\u9879\u76ee\u9a8c\u6536\u7684\u4e00\u7ad9\u5f0f\u5b9e\u9a8c\u5ba4\u89e3\u51b3\u65b9\u6848\uff01\u4e13\u4e1a\u7684\u8bbe\u8ba1\uff0c\u6253\u9020\u7b26\u5408\u4eba\u4f53\u5de5\u5b66\u6807\u51c6\u53c8\u7b26\u5408\u6cd5\u5f8b\u6cd5\u89c4\u7684\u5b9e\u9a8c\u5ba4\u3002\u8ba9\u60a8\u4f53\u9a8c\u66f4\u597d\u7684\u5b9e\u9a8c\u5ba4\u9879\u76ee\u670d\u52a1\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6770\u745e\u601dPCR\u5b9e\u9a8c\u5ba4\u662f\u6307\u7684\u662f\u57fa\u56e0\u6269\u589e\u5b9e\u9a8c\uff0c\u5176\u7279\u70b9\u662f\u80fd\u5c06\u5fae\u91cf\u7684DNA\u5927\u5e45\u589e\u52a0\uff0c\u662f\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u548c\u5b9e\u9a8c\u7684\u5e38\u89c4\u65b9\u6cd5\uff0cPCR\u5b9e […]<\/p>\n","protected":false},"author":1,"featured_media":4977,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,4],"tags":[113,121,132,28],"_links":{"self":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts\/5009"}],"collection":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/comments?post=5009"}],"version-history":[{"count":1,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts\/5009\/revisions"}],"predecessor-version":[{"id":5010,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/posts\/5009\/revisions\/5010"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/media\/4977"}],"wp:attachment":[{"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/media?parent=5009"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/categories?post=5009"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cdjrs.com\/wp-json\/wp\/v2\/tags?post=5009"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}